normal human hepatocyte (hh) cells  (ScienCell)

Journal: Translational Cancer Research

Article Title: Effects of gold nanoparticles on normal hepatocytes in radiation therapy

doi: 10.21037/tcr-21-1855

Figure Lengend Snippet: Effects of AuNPs + radiation combined treatment on the cell proliferation of normal hepatocytes and hepatic stellate cell line. (A) MTT assays were performed using HH cells treated with AuNPs or X-ray irradiation. (B) Transmission electron microscopy images of AuNPs uptake inside the cell. (C) MTT assays were performed using hepatocyte cells (HH: left panel) and hepatic stellate cell line (LX-2: right panel) treated with AuNPs or X-ray irradiation, or a combination of the two treatments. The blue arrows indicate the magnification of AuNPs. MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; HH, human hepatocyte cell.

Article Snippet: Human hepatocyte cells (HH) were obtained from Sciencell Research Laboratories (Catalog #5200; Carlsbad, CA, USA) and cultured in hepatocyte medium supplemented with 5% fetal bovine serum (FBS), 1% penicillin and streptomycin, and 1% hepatocyte growth supplement (all from Sciencell).

Techniques: Irradiation, Transmission Assay, Electron Microscopy

Journal: Translational Cancer Research

Article Title: Effects of gold nanoparticles on normal hepatocytes in radiation therapy

doi: 10.21037/tcr-21-1855

Figure Lengend Snippet: Cell SF of AuNPs + radiation combined treatment of normal hepatocytes. Clonogenic assay of hepatocytes after treatment with AuNPs or X-ray irradiation or a combination of the two treatments. SF, survival fraction; IR, irradiation.

Article Snippet: Human hepatocyte cells (HH) were obtained from Sciencell Research Laboratories (Catalog #5200; Carlsbad, CA, USA) and cultured in hepatocyte medium supplemented with 5% fetal bovine serum (FBS), 1% penicillin and streptomycin, and 1% hepatocyte growth supplement (all from Sciencell).

Techniques: Clonogenic Assay, Irradiation

Journal: Translational Cancer Research

Article Title: Effects of gold nanoparticles on normal hepatocytes in radiation therapy

doi: 10.21037/tcr-21-1855

Figure Lengend Snippet: Effects on apoptosis of AuNPs + radiation combined treatment of hepatocytes. (A) Cells were treated with PI at 48 h post-treatment after each indicated treatment and analyzed by FACS. (B) The indicated antibodies were used for western blotting of hepatocytes treated with AuNPs or X-ray irradiation or a combination of the two treatments. Values represent the means of 3 experiments ± SD. (C) Cells were treated with AuNPs and/or X-ray irradiation or a combination of the two treatments for 24 h. The cell-cycle distribution of subG1 was analyzed quantitatively. *, P<0.05 and **, P<0.01. IR, irradiation; PARP, poly (ADP-ribose) polymerase; PI, propidium iodide; FACS, fluorescence-activated cell sorting.

Article Snippet: Human hepatocyte cells (HH) were obtained from Sciencell Research Laboratories (Catalog #5200; Carlsbad, CA, USA) and cultured in hepatocyte medium supplemented with 5% fetal bovine serum (FBS), 1% penicillin and streptomycin, and 1% hepatocyte growth supplement (all from Sciencell).

Techniques: Western Blot, Irradiation, Fluorescence, FACS

Journal: Translational Cancer Research

Article Title: Effects of gold nanoparticles on normal hepatocytes in radiation therapy

doi: 10.21037/tcr-21-1855

Figure Lengend Snippet: IL-8 concentrations secreted from normal hepatocytes after AuNPs + X-ray irradiation combined treatment. In all therapeutic groups, a decrease in the amount of IL-8 was observed in the radiation alone group. The combination therapy did not lead to significantly lower levels of IL-8 than radiation alone. IL-8, interleukin-8; IR, irradiation.

Article Snippet: Human hepatocyte cells (HH) were obtained from Sciencell Research Laboratories (Catalog #5200; Carlsbad, CA, USA) and cultured in hepatocyte medium supplemented with 5% fetal bovine serum (FBS), 1% penicillin and streptomycin, and 1% hepatocyte growth supplement (all from Sciencell).

Techniques: Irradiation

Journal: Theranostics

Article Title: Inhibition of HSP90β Improves Lipid Disorders by Promoting Mature SREBPs Degradation via the Ubiquitin-proteasome System

doi: 10.7150/thno.36505

Figure Lengend Snippet: Knockdown of HSP90β Improves Lipid Homeostasis. (A and B) The cellular TG and TC levels were measured in human liver HL-7702 hepatocytes transfected with siRNA targeting HSP90β or HSP90α (A) or overexpression (OE) of HSP90β (3.6 times) or HSP90α (4.2 times) (B), the effect of interference or overexpression was verified by western blot. (C) The siRNA treated HL-7702 hepatocytes incubated in medium B for 48 h, and then switched to medium D for another 24 h. The cells then were stained with Nile-Red, which specifically recognizes neutral lipids or filipin, which definitely binds free cholesterol. (D) HL-7702 hepatocytes were incubated in medium B treating with indicated concentrations of siRNA HSP90β for 48 h, and then switched to medium D for another 24 h. Acetic acid sodium salt 1- 14 C was directly added into the medium and incubated for additional 2 h. The radioactive products were identified by comparison with unlabeled standards and visualized with iodine vapor. (E-L) Male C57BL/6J mice (6 weeks) were randomly grouped (10 mice each group). Mice were allowed ad libitum access to water and high fat diet (HFD). After four weeks, mice were intravenously injected with titer of 5×10 9 adenovirus expressing the shRNA targeting HSP90β or the shRNA targeting LacZ. HFD was still administrated to mice for additional 14 days. Then, mice were sacrificed and subjected to various analysis. (E) The total protein from mice liver were prepared, and subjected to immunoblot analysis. Statistical analysis of hepatic HSP90β protein level in panel E normalized to Actin. (F) Food intake during the six week treatment. (G) Body weight measurement during the six weeks treatment. (H) The effect of HSP90β knockdown on serum TG, TC, HDL-c and LDL-c levels. (I) Oil red staining of liver sections. (J) The weight of liver. (K) The TC and TG level in the liver. (L) The TC and TG in the feces were analyzed by GC-MS. (M) Lipid mebabolism related genes expression was detected by q-RT-PCR. Mouse GAPDH was used as the internal control. Error bars are represented as mean ± SEM. Statistical analysis was done with one-way ANOVA (Dunnett's post test) (A, B and C), two-way ANOVA (Bonferroni's test) (F and G), or student's t -test (E, H, J, K, and L). *p < 0.05, **p < 0.01, ***p < 0.001 vs control vector, control adenovirus or control siRNA.

Article Snippet: HH (Human Hepatocytes) cells were purchased from ScienCell Research Laboratories (San Diego, CA, USA) and were cultured in 10 cm 2 flask coated with poly-L-lysine in medium E.

Techniques: Knockdown, Transfection, Over Expression, Western Blot, Incubation, Staining, Comparison, Injection, Expressing, shRNA, Gas Chromatography-Mass Spectrometry, Reverse Transcription Polymerase Chain Reaction, Control, Plasmid Preparation

Journal: Theranostics

Article Title: Inhibition of HSP90β Improves Lipid Disorders by Promoting Mature SREBPs Degradation via the Ubiquitin-proteasome System

doi: 10.7150/thno.36505

Figure Lengend Snippet: Corylin Inhibits de novo Lipids Synthesis by Regulating SREBPs Activity. (A) HL-7702 cells were treated with HSP90 inhibitors with different structure backbone. Only corylin did not cause cytotoxicity. (B) HL7702 cells were depleted of sterols by incubating in medium D for 24 h, and then switched to medium D containing indicated concentration of corylin for 4 h, or corylin (3 μg/ml) for different time, the whole cell extracts underwent immunoblotting with indicated antibodies. (C-D) HL7702 cells were depleted of sterols by incubating in medium D for 24 h, and then switched to medium D containing 3 µg/ml corylin for 4 h. The expression of various genes was analyzed by qRT-PCR. (E) HL7702 cells were depleted of sterols by incubating in medium D for 24 h, and then switched to medium D containing 3 μg/ml corylin or 2 μM lovastatin for 16 h. Acetic acid sodium salt 1- 14 C was directly added into the medium and incubated for additional 2 h. (F) 293T cells were transfected with Myc-HSP90β and HA-Akt for 24 h, after the treatment, the cells were incubated with medium D containing corylin for another 4 h. Cells were lysed and pulled down by Myc antibody. HL-7702 hepatocytes were treated with GSK3β inhibitor SB216763 (10 µM) (G) or CHIR-9902 (10 µM) (H) for 1 h, the cells were switched to medium D supplemented with inhibitors plus vehicle, or 3 µg/ml corylin for 4 h. The whole cell extracts underwent immunoblotting with indicated antibodies. (I) HL-7702 hepatocytes were transfected with GSK3β siRNA for 48 h, after the treatment, the cells were switched to medium D treated with 3 µg/ml corylin for 4 h. (J) HL-7702 hepatocytes were transfected with HA-Akt for 24 h, after the treatment, the cells were switched to medium D treated with 3 µg/ml corylin for 4 h. Whole cell extracts underwent immunoblotting with indicated antibodies. Error bars are represented as mean ± SEM. Statistical analyses were done with one-way ANOVA (Dunnett's post test) (A and C). *p < 0.05, **p < 0.01, ***p < 0.001 vs DMSO.

Article Snippet: HH (Human Hepatocytes) cells were purchased from ScienCell Research Laboratories (San Diego, CA, USA) and were cultured in 10 cm 2 flask coated with poly-L-lysine in medium E.

Techniques: Activity Assay, Concentration Assay, Western Blot, Expressing, Quantitative RT-PCR, Incubation, Transfection